3aED2. Effect of acoustic cavitation on genetically engineered bacteria.

Session: Wednesday Morning, June 18


Author: Sylvia D. S. Kwakye
Location: Dept. of Eng., Swarthmore College, Swarthmore, PA 19081
Author: E. Carr Everbach
Location: Dept. of Eng., Swarthmore College, Swarthmore, PA 19081

Abstract:

The interaction of a complex acoustic cavitation field and suspended cells in vitro has been explored by many previous investigators. Of greatest interest is the effect of collapsing cavitation bubbles on the membrane structure and DNA of cells. By employing E. coli bacteria that have been genetically engineered to emit visible light when they are stressed in proscribed ways, the factors influencing bubble-cell interactions can be better explored. These factors include insonation frequency (1--5 MHz), duty cycle, PRF, initial bubble size distribution, and fluid viscosity. Results from this ongoing study will be presented. [Work supported by NSF.]


ASA 133rd meeting - Penn State, June 1997